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ucp1 abclonal a5857 antibody  (ABclonal Biotechnology)

 
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    Structured Review

    ABclonal Biotechnology ucp1 abclonal a5857 antibody
    Increased energy expenditure in Ccdc92 KO mice on HFD (A-D) Male Ccdc92 KO and littermate WT mice were fed HFD for 12 weeks (A) Food intake/BW, (B) total locomotor activity/BW, (C) O 2 consumption/BW, and (D) energy expenditure/BW were measured using the Comprehensive Lab Animal Monitoring System (CLAMS), WT: n = 5, Ccdc92 KO: n = 7. (E-H) Male Ccdc92 KO and littermate WT mice were fed HFD for 14 weeks (E) Representative images of H&E staining and quantification of adipocyte size in brown adipose tissue (BAT), n = 5/group. Scale bar = 50 μm (F) Representative images of IHC for <t>UCP1</t> in BAT and quantitative analysis, n = 6/group. Scale bar = 50 μm (G) Representative immunoblot and quantification of UCP1, n = 4/group. (H) mRNA expression of lipid catabolism and thermogenesis-related genes in BAT was determined by RT-qPCR, n = 7-8/group. Data are presented as mean ± SEM, ∗p < 0.05; ∗∗p < 0.01. Analysis in A-D and H used two-way ANOVA with Bonferroni correction. Analysis in E, F, and G used unpaired two-tailed t -test.
    Ucp1 Abclonal A5857 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ucp1 abclonal a5857 antibody/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    ucp1 abclonal a5857 antibody - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice"

    Article Title: Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice

    Journal: iScience

    doi: 10.1016/j.isci.2022.105769

    Increased energy expenditure in Ccdc92 KO mice on HFD (A-D) Male Ccdc92 KO and littermate WT mice were fed HFD for 12 weeks (A) Food intake/BW, (B) total locomotor activity/BW, (C) O 2 consumption/BW, and (D) energy expenditure/BW were measured using the Comprehensive Lab Animal Monitoring System (CLAMS), WT: n = 5, Ccdc92 KO: n = 7. (E-H) Male Ccdc92 KO and littermate WT mice were fed HFD for 14 weeks (E) Representative images of H&E staining and quantification of adipocyte size in brown adipose tissue (BAT), n = 5/group. Scale bar = 50 μm (F) Representative images of IHC for UCP1 in BAT and quantitative analysis, n = 6/group. Scale bar = 50 μm (G) Representative immunoblot and quantification of UCP1, n = 4/group. (H) mRNA expression of lipid catabolism and thermogenesis-related genes in BAT was determined by RT-qPCR, n = 7-8/group. Data are presented as mean ± SEM, ∗p < 0.05; ∗∗p < 0.01. Analysis in A-D and H used two-way ANOVA with Bonferroni correction. Analysis in E, F, and G used unpaired two-tailed t -test.
    Figure Legend Snippet: Increased energy expenditure in Ccdc92 KO mice on HFD (A-D) Male Ccdc92 KO and littermate WT mice were fed HFD for 12 weeks (A) Food intake/BW, (B) total locomotor activity/BW, (C) O 2 consumption/BW, and (D) energy expenditure/BW were measured using the Comprehensive Lab Animal Monitoring System (CLAMS), WT: n = 5, Ccdc92 KO: n = 7. (E-H) Male Ccdc92 KO and littermate WT mice were fed HFD for 14 weeks (E) Representative images of H&E staining and quantification of adipocyte size in brown adipose tissue (BAT), n = 5/group. Scale bar = 50 μm (F) Representative images of IHC for UCP1 in BAT and quantitative analysis, n = 6/group. Scale bar = 50 μm (G) Representative immunoblot and quantification of UCP1, n = 4/group. (H) mRNA expression of lipid catabolism and thermogenesis-related genes in BAT was determined by RT-qPCR, n = 7-8/group. Data are presented as mean ± SEM, ∗p < 0.05; ∗∗p < 0.01. Analysis in A-D and H used two-way ANOVA with Bonferroni correction. Analysis in E, F, and G used unpaired two-tailed t -test.

    Techniques Used: Activity Assay, Staining, Western Blot, Expressing, Quantitative RT-PCR, Two Tailed Test


    Figure Legend Snippet:

    Techniques Used: Derivative Assay, Virus, Recombinant, Lysis, Extraction, Immunoprecipitation, Magnetic Beads, Protease Inhibitor, Luciferase, SYBR Green Assay, Membrane, Bradford Assay, Saline, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Sequencing, RNA Sequencing, Software



    Similar Products

    ucp1 abclonal a5857 antibody  (ABclonal Biotechnology)
    90
    ABclonal Biotechnology ucp1 abclonal a5857 antibody
    Increased energy expenditure in Ccdc92 KO mice on HFD (A-D) Male Ccdc92 KO and littermate WT mice were fed HFD for 12 weeks (A) Food intake/BW, (B) total locomotor activity/BW, (C) O 2 consumption/BW, and (D) energy expenditure/BW were measured using the Comprehensive Lab Animal Monitoring System (CLAMS), WT: n = 5, Ccdc92 KO: n = 7. (E-H) Male Ccdc92 KO and littermate WT mice were fed HFD for 14 weeks (E) Representative images of H&E staining and quantification of adipocyte size in brown adipose tissue (BAT), n = 5/group. Scale bar = 50 μm (F) Representative images of IHC for <t>UCP1</t> in BAT and quantitative analysis, n = 6/group. Scale bar = 50 μm (G) Representative immunoblot and quantification of UCP1, n = 4/group. (H) mRNA expression of lipid catabolism and thermogenesis-related genes in BAT was determined by RT-qPCR, n = 7-8/group. Data are presented as mean ± SEM, ∗p < 0.05; ∗∗p < 0.01. Analysis in A-D and H used two-way ANOVA with Bonferroni correction. Analysis in E, F, and G used unpaired two-tailed t -test.
    Ucp1 Abclonal A5857 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ucp1 abclonal a5857 antibody/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    ucp1 abclonal a5857 antibody - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Increased energy expenditure in Ccdc92 KO mice on HFD (A-D) Male Ccdc92 KO and littermate WT mice were fed HFD for 12 weeks (A) Food intake/BW, (B) total locomotor activity/BW, (C) O 2 consumption/BW, and (D) energy expenditure/BW were measured using the Comprehensive Lab Animal Monitoring System (CLAMS), WT: n = 5, Ccdc92 KO: n = 7. (E-H) Male Ccdc92 KO and littermate WT mice were fed HFD for 14 weeks (E) Representative images of H&E staining and quantification of adipocyte size in brown adipose tissue (BAT), n = 5/group. Scale bar = 50 μm (F) Representative images of IHC for UCP1 in BAT and quantitative analysis, n = 6/group. Scale bar = 50 μm (G) Representative immunoblot and quantification of UCP1, n = 4/group. (H) mRNA expression of lipid catabolism and thermogenesis-related genes in BAT was determined by RT-qPCR, n = 7-8/group. Data are presented as mean ± SEM, ∗p < 0.05; ∗∗p < 0.01. Analysis in A-D and H used two-way ANOVA with Bonferroni correction. Analysis in E, F, and G used unpaired two-tailed t -test.

    Journal: iScience

    Article Title: Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice

    doi: 10.1016/j.isci.2022.105769

    Figure Lengend Snippet: Increased energy expenditure in Ccdc92 KO mice on HFD (A-D) Male Ccdc92 KO and littermate WT mice were fed HFD for 12 weeks (A) Food intake/BW, (B) total locomotor activity/BW, (C) O 2 consumption/BW, and (D) energy expenditure/BW were measured using the Comprehensive Lab Animal Monitoring System (CLAMS), WT: n = 5, Ccdc92 KO: n = 7. (E-H) Male Ccdc92 KO and littermate WT mice were fed HFD for 14 weeks (E) Representative images of H&E staining and quantification of adipocyte size in brown adipose tissue (BAT), n = 5/group. Scale bar = 50 μm (F) Representative images of IHC for UCP1 in BAT and quantitative analysis, n = 6/group. Scale bar = 50 μm (G) Representative immunoblot and quantification of UCP1, n = 4/group. (H) mRNA expression of lipid catabolism and thermogenesis-related genes in BAT was determined by RT-qPCR, n = 7-8/group. Data are presented as mean ± SEM, ∗p < 0.05; ∗∗p < 0.01. Analysis in A-D and H used two-way ANOVA with Bonferroni correction. Analysis in E, F, and G used unpaired two-tailed t -test.

    Article Snippet: For immunostaining, mouse tissue sections embedded in paraffin were incubated with primary antibodies, including CD44 (BioLegend #103002, 1:200), CD68 (Santa Cruz #sc-20060, 1:200), Perilipin (Novus #NB100-60554, 1:200), or UCP1 (ABclonal, A5857, 1:200) overnight at 4°C, followed by incubation with a fluorescent-dye (Alexa Fluor 594- or Alexa Fluor 488; 1:1,000)-conjugated or enzyme (Alkaline phosphatase, 1:1,000)-conjugated secondary antibody.

    Techniques: Activity Assay, Staining, Western Blot, Expressing, Quantitative RT-PCR, Two Tailed Test

    Journal: iScience

    Article Title: Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice

    doi: 10.1016/j.isci.2022.105769

    Figure Lengend Snippet:

    Article Snippet: For immunostaining, mouse tissue sections embedded in paraffin were incubated with primary antibodies, including CD44 (BioLegend #103002, 1:200), CD68 (Santa Cruz #sc-20060, 1:200), Perilipin (Novus #NB100-60554, 1:200), or UCP1 (ABclonal, A5857, 1:200) overnight at 4°C, followed by incubation with a fluorescent-dye (Alexa Fluor 594- or Alexa Fluor 488; 1:1,000)-conjugated or enzyme (Alkaline phosphatase, 1:1,000)-conjugated secondary antibody.

    Techniques: Derivative Assay, Virus, Recombinant, Lysis, Extraction, Immunoprecipitation, Magnetic Beads, Protease Inhibitor, Luciferase, SYBR Green Assay, Membrane, Bradford Assay, Saline, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Sequencing, RNA Sequencing, Software